Mixed-charge pseudo-zwitterionic copolymer brush as broad spectrum antibiofilm coating

Zwitterionic polymers are classical antifouling but they require specialized monomers that have cationic and anionic charges integrated into a single monomer. Herein, we show pseudo-zwitterionic copolymers synthesized from mixture of 2 each having opposite polarity has excellent antibiofilm efficacy. We discovered new mixed-charge copolymer brush (#1-A) oppositely charged monomers, the SPM (3-Sulfopropyl methacrylate) AMPTMA ((3-Acrylamidopropyl) trimethylammonium chloride), achieves broad spectrum in vitro effect greater than 99% reductions against all six Gram-positive Gram-negative bacteria tested. In murine subcutaneous wound catheter infection models, #1-A good long-term anti-biofilm efficacy MRSA Pseudomonas aeruginosa 3.41 3.19 orders respectively, outperforming previous coatings. method to choose cationic/anionic pair combination form best coating by exploiting solution polymerization kinetics disparity between monomers. also showed is softer higher hydration zwitterionic polymer. This study shows possibility achieving potent combining readily available singly